General Hospital "Dr. Manuel Gea Gonzalez" 1 article published in JoVE Biology Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples Eduardo Delgadillo-Tellez1, Beatriz Zavaleta-Villa1, Daniel Atilano-López2, Rigoberto Hernández-Castro3, Angélica Olivo-Díaz1, Erika Margarita Carrillo-Casas1 1Department of Molecular Biology and Histocompatibility, General Hospital “Dr. Manuel Gea González”, 2Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty, UNAM, 3Department of Ecology of Pathogens, General Hospital “Dr. Manuel Gea González” In this study, a dot-blot application was designed to detect Leptospira from the three main clades in water samples. This method allows for the identification of minimal DNA quantities specifically targeted by a digoxigenin-labeled probe, easily detected by an anti-digoxigenin antibody. This approach is a valuable and satisfactory tool for screening purposes.
